Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size - Video | Study.com
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The following picture shows the ethidium bromidestained bands revealed by gel electrophoresis of two different DNA samples digested with two different restriction enzymes. One of the DNAs is human genomic DNA, the other is the small genome of a bacteriophage (bacterial virus) that infects E. coli cells. One of the restriction enzymes is E c o RI(5^' G^∧ A ATTC 3^') ; the other is HpaII(5^' C^∧ CGG 3^') . For each of the four lanes on the gel (A-D), identify which of the two DNA samples was analy
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Ethidium bromide is a compound commonly used to visualize DNA during gel electrophoresis, though its use has been phased out due to toxicity and mutagenic concerns. This compound stains DNA by intercalating. Explain what this means in the context of DNA base pairs.
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